May 2016: New methods certified NF VALIDATION !

As of May 18th 2016, 123 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 6 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are also validated according to an NF VALIDATION specific protocol.

During its meeting held on May 17th and 18th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of 3M Molecular Detection Assay 2 – Listeria (3M Health Care) for the detection of Listeria spp. in all human food and industrial environmental samples according to the validation protocol ISO/FDIS 16140-2:2016.

– The validation renewal (without modification) of Simple Method for Salmonella method (bioMérieux) according to the validation protocol EN/ISO 16140:2003.

Others files are currently in process.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

June 30th and July 1st, September 29th and 30th, November 24th and 25th 2015.

New validations in food microbiology as of March 2016

As of March 18th 2016, 122 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 5 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are validated according to an NF VALIDATION specific protocol.

During its meeting held on March 17th and 18th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of ANSR for Listeria monocytogenes (NEOGEN Europe Ltd) for the detection of Listeria spp. in all human food and industrial environmental samples according to the validation protocol ISO/FDIS 16140-2:2015.

– The validation extensions of the following methods: VIDAS Campylobacter and Gélose CampyFood (bioMérieux) for the possibility of using VITEK MS test for the confirmation of presumptive Campylobacter spp., Thermo Scientific SureTect Listeria monocytogenes PCR Assay (OXOID Thermo Fisher Scientific) for the possibility of using ABI 7500 Fast RT-PCR instrument and its RapidFinder Express 2.0 software for results analysis and interpretation of for meat and dairy products, and TRANSIA Plate Salmonella Gold (BioControl Systems) for a new enrichment protocol in “RVS + Novobiocin” broth.

– The validation renewal (without modification) of the following methods: VIDAS Up Listeria (bioMérieux), TRANSIA Plate Listeria monocytogenes (BioControl Systems), BAX System PCR Assay for E. coli O157:H7 MP, BAX System PCR Assay Genus Listeria 24E and BAX System PCR Assay Listeria monocytogenes 24E (DuPont Nutrition & Health), iQ-check E.coli O157:H7, iQ-Check Salmonella II and RAPID’Sakazakii (BIO-RAD), RIDASCREEN Salmonella (R-Biopham).

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

News on NF VALIDATION side for water application as of March 2016

As of March 11th, 2016, 15 methods for microbiological analysis in water are certified NF VALIDATION. The  qPCR kits for Legionella qualitative and quantitative detection are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a technical protocol, developed by AFNOR Certification and based on the comparison of an alternative method to a standard method.

During its meeting held on March 11th, 2016, the NF VALIDATION “Technical Board – Water General protocol” approved by vote :

– The validation of Pseudalert/Quanti-Tray method (IDEXX Laboratories) for the enumeration of Pseudomonas aeruginosa in pool water and waters for human consumption.

– The validation renewals for RAPID’P. aeruginosa Agar method (Bio-Rad) and REBECCA CF WATERS method (bioMérieux).

Next meeting for water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Wednesday 1st of June, 2016

NF VALIDATION: some news in food microbiology

As of January 29th 2015, 121 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 4 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are validated according to an NF VALIDATION specific protocol.

During its meeting held on January 28th and 29th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of ANSR for Listeria (NEOGEN Europe Ltd) for the detection of Listeria spp. in all human food products and industrial environmental samples according to the new protocol ISO/FDIS 16140-2 protocol.

– The validation extensions of the following methods: EASY STAPH (SOLABIA) for all human food products , BAX System PCR Assay for E. coli O157:H7 and BAX System PCR Assay for Salmonella spp. (DuPont Nutrition & Health) for the use of the BAX Sytem X5 with its software 1.0, Thermo Scientific SureTect Escherichia coli O157:H7 (OXOID Thermo Fisher Scientific) for the use of the ABI 7500 Fast RT-PCR system with the RapidFinder Express 2.0 software, and VIDAS LMO2 (bioMérieux) for a new enrichment protocol at 30°C using LPT broth for the analysis of meat products, dairy products and seafood products.

– The validation renewal (without modification) of the Delvotest T method (DSM Food specialties).

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

March 17th and 18th, May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

NF VALIDATION: News in the water domain

As of December 17th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

As of December 17th, 2015, the NF VALIDATION “Technical Board – Water PCR Legionella” approved by vote the validation renewal of the methods GeneDisc Legionella spp. and GeneDisc Legionella pneumophila (PALL GeneDisc Technologies), with an extension of their scope to the qualitative detection. An extension has been also granted for these methods for the possibility of using GeneDisc Ultra-Purifier and GeneDisc Plate Legionella ID.

Other validation files are currently submitted to NF VALIDATION experts’ review.

Next meetings in water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Friday 11th of March, 2016

NF VALIDATION : 124 methods certified in food microbiology

As of December 03th 2015, 121 alternative methods for microbiological food analysis are certified NF VALIDATION according to the NF EN ISO 16140 standard protocol, and 3 for antibiotic detection in food according to a specific validation protocol.

During its meeting held on December 03rd and 04th 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation according to the new protocol ISO/FDIS 16140-2 (2015) of Thermo Scientific SureTect Cronobacter species PCR Assay (OXOID Thermo Fisher Scienific) for the detection of Cronobacter spp. in infant formula (with or without probiotics) and related industrial environmental samples, and EASY STAPH (SOLABIA) for the enumeration of coagulase-positive Staphylococci in meat products and poultry, dairy products and seafood.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”: January 28th and 29th, March 17th and 18th, May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th 2016.

NF VALIDATION news for the water application as of October 2015

As of October 14th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

During its meeting held on October 14th, 2015, the NF VALIDATION “Technical Board – Water PCR Legionella” approved by vote the validation renewal of the methods iQ-Check Legionella spp. and iQ-Check Legionella pneumophila (Bio-Rad), with an extension of their scope to the detection.

Other validation files are currently submitted to NF VALIDATION experts’ review.

Next meetings in water domain:

– NF VALIDATION “Technical Board – Water PCR Legionella” : Wednesday 9th of December, 2015

– NF VALIDATION “Technical Board – Water General protocol” : Friday 11th of March, 2016

Food microbiology: 122 methods certified NF VALIDATION

As of October 14th 2015, 119 alternative methods for microbiological food analysis are certified NF VALIDATION according to the NF EN ISO 16140 standard protocol, and 3 for antibiotic detection in food according to a specific validation protocol.

During its meeting held on October 08th and 09th 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of Solus E. coli O157 ELISA (Solus Scientific) for the detection of Salmonella spp. in raw beef meats (seasoned or not), dairy products and raw milks, vegetables and production environmental samples.

– The validation extension of VIDAS Easy Salmonella (bioMérieux) for the use of VITEK MS test for the confirmation of presumptive Salmonella spp.

– The validation renewal (without modification) of the following methods: RAPID’E. coli O157:H7 (BIO-RAD), REBECCA BASE or REBECCA + EB (bioMérieux), REBECCA + EB (bioMérieux).

– The validation renewal (with extension) of VIDAS UP Salmonella (bioMérieux), for the use of VITEK MS test for the confirmation of presumptive Salmonella spp, and also an update of the validation file according to the new ISO 16140-2 protocol.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

– 2015: December 3rd and 4th 2015.

2016: January 28th and 29th, March 17th and 18th, May 12th and 13th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

What’s up on July 2015 on the water validation side ?

As of July 06th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

During its meeting held the 3rd July 2015, the Technical Board – Water General protocol examined validation files of alternative methods currently submitted to the NF VALIDATION certification process.

Next meetings in water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Monday 23rd of November, 2015 and Friday 11th of March, 2016.

– NF VALIDATION “Technical Board – Water PCR Legionella” : Wednesday 14th of October, 2015.

NF VALIDATION: July 2015 news for food microbiology validation

As of June 06th 2015, 121 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 :2003 standard protocol requirements.

During its meeting held on July 2nd and 3rd 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation extension of the following methods: BACGene Salmonella spp (Eurofins GeneScan) for a new protocol with 50g up to 375g sample sizes for milk powders and infant formula with and without probiotics; RAPID’Salmonella /Agar (BIO-RAD) for a new protocol with 50g up to 375g sample sizes for milk powders, including infant formula with and without probiotics and related dehydrated dairy ingredients; ThermoScientific SureTect E. coli O157:H7 PCR Assay (OXOID Thermo Fisher Scientific) for an updated version of the related kit file of the SureTect software.

– The validation renewal of the following methods: TRANSIA PLATE Listeria (BioControl Systems), ADIAFOOD Escherichia coli O157:H7 (bioMérieux), iQ-Check Listeria spp. (BIO-RAD), MicroSEQ Listeria spp. and MicroSEQ Listeria monocytogenes (Life Technologies), Reveal 2.0 Salmonella (Neogen Europe), Salmonella Precis (OXOID Thermo Fisher Scientific), IRIS Salmonella, SESAME Salmonella TEST and COMPASS Listeria Agar Enumeration (SOLABIA).

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”:

2015: October 8th and 9th, December 3rd and 4th 2015.

2016: January 28th and 29th, March 17th and 18th, May 12th and 13th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.