What’s up on July 2015 on the water validation side ?

As of July 06th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

During its meeting held the 3rd July 2015, the Technical Board – Water General protocol examined validation files of alternative methods currently submitted to the NF VALIDATION certification process.

Next meetings in water domain:

- NF VALIDATION “Technical Board – Water General protocol” : Monday 23rd of November, 2015 and Friday 11th of March, 2016.

- NF VALIDATION “Technical Board – Water PCR Legionella” : Wednesday 14th of October, 2015.

NF VALIDATION: July 2015 news for food microbiology validation

As of June 06th 2015, 121 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 :2003 standard protocol requirements.

During its meeting held on July 2nd and 3rd 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

- The validation extension of the following methods: BACGene Salmonella spp (Eurofins GeneScan) for a new protocol with 50g up to 375g sample sizes for milk powders and infant formula with and without probiotics; RAPID’Salmonella /Agar (BIO-RAD) for a new protocol with 50g up to 375g sample sizes for milk powders, including infant formula with and without probiotics and related dehydrated dairy ingredients; ThermoScientific SureTect E. coli O157:H7 PCR Assay (OXOID Thermo Fisher Scientific) for an updated version of the related kit file of the SureTect software.

- The validation renewal of the following methods: TRANSIA PLATE Listeria (BioControl Systems), ADIAFOOD Escherichia coli O157:H7 (bioMérieux), iQ-Check Listeria spp. (BIO-RAD), MicroSEQ Listeria spp. and MicroSEQ Listeria monocytogenes (Life Technologies), Reveal 2.0 Salmonella (Neogen Europe), Salmonella Precis (OXOID Thermo Fisher Scientific), IRIS Salmonella, SESAME Salmonella TEST and COMPASS Listeria Agar Enumeration (SOLABIA).

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”:

2015: October 8th and 9th, December 3rd and 4th 2015.

2016: January 28th and 29th, March 17th and 18th, May 12th and 13th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

NF VALIDATION: 15 methods certified in Water domain

As of June 09th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

During its meeting held on June 08th, 2015, the NF VALIDATION “Technical Board – Water General protocol” approved by vote:

- The validation renewal of the method RAPID’E. coli 2 + Supplement for water testing (Bio-Rad) for the enumeration of E. coli ß D-glucuronidase positive and coliform bacteria ß D-galactosidase positive in waters for human consumption.

Others analysis methods are currently following the NF VALIDATION process.

Next meetings in water domain:

- NF VALIDATION “Technical Board – Water General protocol” : Friday 3rd of July, 2015.

- NF VALIDATION “Technical Board - WaterPCR Legionella” : Wednesday 14th of October, 2015

Food microbiology: 121 methods certified NF VALIDATION

As of June 05th 2015, 121 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 standard protocol requirements.

During its meeting held on May 21st 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

- The validation of a new method: RHAPSODY (SOLABIA) for the enumeration of Pseudomonas spp in meat products and dairy products.

- The validation extension to the categories “feed products (25g)”, “pet foods (375g)” and “production environment samples (excluding primary production environment)” for BACGene Salmonella spp (Eurofins GeneScan).

- The validation renewal of the following methods: TAG 24 Salmonella (BioControl Systems), IBISA (bioMérieux) and TaqMan Salmonella enterica (Life Technologies).

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”: July 2nd and 3rd, October 8th and 9th, December 3rd and 4th 2015.

120 methods certified NF VALIDATION in food microbiology

As of March 26th 2015, 120 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 standard protocol requirements.

During its meeting held on March 26th 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

- The validation of a new method: DuPont BAX System Real-Time PCR Assay for Salmonella spp (DuPont Qualicon) for the detection of Salmonella spp in meat products, egg products, vegetables and seafood, and pet food; BACGene Salmonella spp (Eurofins GeneScan) for the detection of Salmonella spp in all human food products and Thermo Scientific SureTect Escherichia coli O157:H7 PCR Assay (Oxoid Thermo Fisher Scientific) for the detection of E. coli O157:H7 in raw beef.

- The validation renewal of the following methods: chromID Lmono Agar (bioMérieux) and OXOID Listeria Rapid Test (Oxoid Thermo Fisher Scientific).

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”: May 21st and 22th, July 2nd and 3rd, October 8th and 9th, December 3rd and 4th 2015

A new validation for water microbiology in February 2015

As of 27th February 2015, 15 methods for microbiological water analysis are certified NF VALIDATION. The methods for quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification that compares the alternative method to a standard method.

During their meeting held on 26th February 2015:

The Water Technical Board “General protocol” validated a new method: Enterolert-E/Quanti-Tray or Quanti-Tray 2000 for the enumeration of intestinal enterococci in bathing waters. Other validation files are currently submitted to examination.

The Water Technical Board “PCR Legionella” revised the applicable validation protocol, in order to integrate the requirements of the new NF T90-471 standard (issuing very soon). The validation protocol draft and its transition modalities will be submitted to the approval of the NF Validation Experts Committee, before being published.

Next meeting of Water Technical Board “General protocol” : 8th June 2015.

Next meeting of Water Technical Board “PCR Legionella”: 14th September 2015.

NF VALIDATION: News on the side of Food microbioloogy validation

As of February 6th 2015, 117 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 :2003 standard protocol requirements.

During its meeting held on February 6th 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

- The validation renewals of the following methods: 3M Petrifilm Staph Express Test System A and B (3M Health Care), AccuProbe Listeria monocytogenes (bioMérieux) and MicroSEQ E. coli O157: H7 (Life Technologies).

- The validation extensions to the categories “Pet food” and “Industrial environment samples” for the Test 3M Petrifilm Coliforms Count Plate(A), Test 3M Petrifilm Aerobic Count Plate and Test 3M Petrifilm Select E. coli  Count Plate, and to the category “Industrial environment samples” for the Test 3M Petrifilm Enterobacteriaceae Count Plate(3M Health Care).

Others files are under the process of validation.

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”: March 26th and 27th, May 21st and 22th, July 2nd and 3rd, October 8th and 9th, December 3rd and 4th.

NF VALIDATION certificates are changing

Still available in French and English, the certificate is given a fresh new look and its content is simplified. Our objective: accelerate the issuance of your certificate and increase your satisfaction! The validation data remain documented and available for your convenience in the summarized validation study reports.

The news on the water validation side

As of 8th January 2015, 14 methods for microbiological water analysis are certified NF VALIDATION. The methods for quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification that compares the alternative method to a standard method.

During its meeting held the 7th November 2014, the Water Technical Board “General protocol” validated, for Colilert-18 applicable to bathing water, the possibility to use either the Quanti-Tray or the Quanti-Tray 2000. Other validation files are currently submitted to examination.

A new version of the NF T90-471 standard is going to be published. A new meeting of the Water Technical Board “PCR Legionella” will be held very soon, in order to estimate the impact of the new standard on the validation protocol and validation files. Modalities of transition to integrate new requirements will be defined in the context of NF VALIDATION mark.

Next meeting of both Water Technical Boards “General protocol” and “PCR Legionella”: 26th February 2015.

NF VALIDATION: New validations for food microbiology scheme

As of November 28th 2014, 117 microbiological methods in the food industry including 3 antibiotic detection methods are certified NF VALIDATION. All the microbiological methods are validated according to the NF EN ISO 16140 standard protocol requirements.

During its meeting held on November 27th and 28th 2014, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

- The validation of a new method: ESIA One Day (bioMérieux) for detection of Cronobacter spp in infant formulas (liquids and powders), milk powders and dehydrated complements of infant formulas, matrices used in the composition of infant formulas (liquids and powders) and soy milk, environmental samples from milk powders and infant formulas industries.

- The validation renewal of the following methods: 3M Petrifilm High Sensitivity Coliform Count Plate (3M Health Care), chromeID Coli Agar at 37°C (bioMérieux), chromeID Coli Agar at 44°C(bioMérieux), chromeID Coli Agar for coliforms at 37°C (bioMérieux), RAPID’Listeria spp (BIO-RAD), BAX System PCR Assay Salmonella spp (DuPont Qualicon) and COMPASS Listeria AGAR (SOLABIA).

Next meetings of NF VALIDATION “Technical Board – Food Microbiology”:

- 2015 : February 5th and 6th, March 26th and 27th, May 21st and 22th, July 2nd and 3rd, October 8th and 9th, December 3rd and 4th