Category Archives: News

Transition procedures ISO 16140-2

The ISO 16140-2 standard

The validation protocol for alternative analysis methods changed in June 2016, with the publication of the ISO 16140-2 standard.

This revision aims to better take into account market expectations and the harmonisation of requirements between Europe and North America. Continue reading

NF VALIDATION (Food): Technical Board of september 2016

As of September 30th 2016, 126 alternative methods for microbiological food analysis are certified NF VALIDATION : 114 are validated according to the protocol EN/ISO 16140:2003 and 12 according to the protocol EN/ISO 16140-2:2016. Two methods for the detection of antibiotics residues in food are validated according to the NF VALIDATION specific protocol.

During its meeting held on September 29th and 30th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validations (according to the protocol NF EN ISO 16140-2:2016) of two methods: 3M Molecular Detection Assay 2 – Listeria monocytogenes (3M Health Care) for the detection of Listeria monocytogenes in all human food and production environmental samples, GENE-UP Listeria spp. (bioMérieux) for the detection of Listeria spp. (excluding Listeria grayi) in meat and dairy products.

– The following validation extensions: scope of GENE-UP Salmonella (bioMérieux) extended to “all human food products”; validation of a new protocol specific to “composite foods” for LUMIPROBE 24 Listeria monocytogenes (Europrobe).

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”: 2016: November 24th and 25th. 2017: January 26th and 27th, March 23rd and 24th, May 18th and 19th, July 3rd and 4th, September 28th and 29th, November 23th and 24th. 2018: January 25th and 26th, March 22th and 23rd, July 2nd and 3rd.

NF VALIDATION (Food): Technical Board of July 2016

As of July 6th 2016, 125 alternative methods for microbiological food analysis are certified NF VALIDATION : 115 are validated according to the protocol EN/ISO 16140:2003 and 10 according to the protocol ISO 16140-2:2016. Two methods for the detection of antibiotics residues in food are validated according to the NF VALIDATION specific protocol.

During its meeting held on June 30th and July 1st 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validations according to the protocol ISO 16140-2:2016 of GENE-UP Salmonella (bioMérieux) for the detection of Salmonella spp. in meat products, and ready-to-eat and ready-to-reheat food products, LESS Plus for Listeria + ANSR for Listeria or culture media and LESS Plus for Listeria monocytogenes + ANSR for Listeria monocytogenes or culture media (NEOGEN Europe Ltd) for the detection of Listeria spp. or Listeria monocytogenes in all human food products and production environmental samples.

– The validation extensions of the following methods for OXOID Thermo Fisher Scientific: Thermo Scientific SureTect Listeria species PCR Assay and Thermo Scientific SureTect Listeria monocytogenes PCR Assay for the use of ABI 7500 Fast instrument and its RapidFinder Express software V2.0, Thermo Scientific SureTect Cronobacter species PCR Assay for a new protocol for powdered infant formula (300g) and the use of ABI 7500 Fast instrument and its RapidFinder Express software V2.0, Thermo Scientific SureTect Salmonella species PCR Assay for a new enrichment protocol for dairy products and the use of ABI 7500 Fast instrument and its RapidFinder Express software V2.0.

– The validation renewals according to the protocol EN/ISO 16140:2003 of 3M Petrifilm Rapid Coliform Count Plate, 3M Petrifilm Select E. coli Count Plate, 3M Molecular Detection Assay for Salmonella, 3M Molecular Detection Assay E. coli O157 (3M Health Care), ALOA One Day (bioMérieux). For VIDAS Listeria monocytogenes II (LMO2) (bioMérieux), the validation renewal has been performed according to the protocol ISO 16140-2:2016.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”: 2016: September 29th and 30th, November 24th and 25th. 2017: January 26th and 27th, March 23rd and 24th, May 18th and 19th, July 3rd and 4th, September 28th and 29th, November 23th and 24th. 2018: January 25th and 26th, March 22th and 23th, July 2nd and 3rd.

NF VALIDATION: News as of June 2016 for the water domain

As of June 1st, 2016, 15 methods for microbiological analysis in water are certified NF VALIDATION. The qPCR kits for Legionella qualitative and quantitative detection are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a technical protocol, developed by AFNOR Certification and based on the comparison of an alternative method to a standard method.

During its meeting held on June 1st, 2016, the NF VALIDATION “Technical Board – Water General protocol” approved by vote the validation renewals of the following methods: MWY Legionella Agar (bioMérieux), Colilert-18 / Quanti-Tray or Quanti-Tray 2000 – Bathing waters (IDEXX Laboratories) and COMPASS cc Agar (SOLABIA).

Next meeting for water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Wednesday 19th of October, 2016

May 2016: New methods certified NF VALIDATION !

As of May 18th 2016, 123 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 6 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are also validated according to an NF VALIDATION specific protocol.

During its meeting held on May 17th and 18th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of 3M Molecular Detection Assay 2 – Listeria (3M Health Care) for the detection of Listeria spp. in all human food and industrial environmental samples according to the validation protocol ISO/FDIS 16140-2:2016.

– The validation renewal (without modification) of Simple Method for Salmonella method (bioMérieux) according to the validation protocol EN/ISO 16140:2003.

Others files are currently in process.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

June 30th and July 1st, September 29th and 30th, November 24th and 25th 2015.

New validations in food microbiology as of March 2016

As of March 18th 2016, 122 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 5 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are validated according to an NF VALIDATION specific protocol.

During its meeting held on March 17th and 18th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of ANSR for Listeria monocytogenes (NEOGEN Europe Ltd) for the detection of Listeria spp. in all human food and industrial environmental samples according to the validation protocol ISO/FDIS 16140-2:2015.

– The validation extensions of the following methods: VIDAS Campylobacter and Gélose CampyFood (bioMérieux) for the possibility of using VITEK MS test for the confirmation of presumptive Campylobacter spp., Thermo Scientific SureTect Listeria monocytogenes PCR Assay (OXOID Thermo Fisher Scientific) for the possibility of using ABI 7500 Fast RT-PCR instrument and its RapidFinder Express 2.0 software for results analysis and interpretation of for meat and dairy products, and TRANSIA Plate Salmonella Gold (BioControl Systems) for a new enrichment protocol in “RVS + Novobiocin” broth.

– The validation renewal (without modification) of the following methods: VIDAS Up Listeria (bioMérieux), TRANSIA Plate Listeria monocytogenes (BioControl Systems), BAX System PCR Assay for E. coli O157:H7 MP, BAX System PCR Assay Genus Listeria 24E and BAX System PCR Assay Listeria monocytogenes 24E (DuPont Nutrition & Health), iQ-check E.coli O157:H7, iQ-Check Salmonella II and RAPID’Sakazakii (BIO-RAD), RIDASCREEN Salmonella (R-Biopham).

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

News on NF VALIDATION side for water application as of March 2016

As of March 11th, 2016, 15 methods for microbiological analysis in water are certified NF VALIDATION. The  qPCR kits for Legionella qualitative and quantitative detection are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a technical protocol, developed by AFNOR Certification and based on the comparison of an alternative method to a standard method.

During its meeting held on March 11th, 2016, the NF VALIDATION “Technical Board – Water General protocol” approved by vote :

– The validation of Pseudalert/Quanti-Tray method (IDEXX Laboratories) for the enumeration of Pseudomonas aeruginosa in pool water and waters for human consumption.

– The validation renewals for RAPID’P. aeruginosa Agar method (Bio-Rad) and REBECCA CF WATERS method (bioMérieux).

Next meeting for water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Wednesday 1st of June, 2016

NF VALIDATION: some news in food microbiology

As of January 29th 2015, 121 alternative methods for microbiological food analysis are certified NF VALIDATION (among which 117 validated according to the standard protocol EN/ISO 16140:2003 and 4 methods validated according to the standard project ISO/FDIS 16140-2:2015). Three methods for the detection of antibiotics residues in food are validated according to an NF VALIDATION specific protocol.

During its meeting held on January 28th and 29th 2016, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation of ANSR for Listeria (NEOGEN Europe Ltd) for the detection of Listeria spp. in all human food products and industrial environmental samples according to the new protocol ISO/FDIS 16140-2 protocol.

– The validation extensions of the following methods: EASY STAPH (SOLABIA) for all human food products , BAX System PCR Assay for E. coli O157:H7 and BAX System PCR Assay for Salmonella spp. (DuPont Nutrition & Health) for the use of the BAX Sytem X5 with its software 1.0, Thermo Scientific SureTect Escherichia coli O157:H7 (OXOID Thermo Fisher Scientific) for the use of the ABI 7500 Fast RT-PCR system with the RapidFinder Express 2.0 software, and VIDAS LMO2 (bioMérieux) for a new enrichment protocol at 30°C using LPT broth for the analysis of meat products, dairy products and seafood products.

– The validation renewal (without modification) of the Delvotest T method (DSM Food specialties).

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”:

March 17th and 18th, May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th.

NF VALIDATION: News in the water domain

As of December 17th, 2015, 15 methods for microbiological analysis in water are certified NF VALIDATION. The tests of quantification of Legionella using PCR are certified according to a protocol based on NF T90-471 and ISO/TS 12869 standards. The other methods are certified according to a specific technical protocol, developed by AFNOR Certification, that compares the alternative method to a standard method for a defined targeted strain.

As of December 17th, 2015, the NF VALIDATION “Technical Board – Water PCR Legionella” approved by vote the validation renewal of the methods GeneDisc Legionella spp. and GeneDisc Legionella pneumophila (PALL GeneDisc Technologies), with an extension of their scope to the qualitative detection. An extension has been also granted for these methods for the possibility of using GeneDisc Ultra-Purifier and GeneDisc Plate Legionella ID.

Other validation files are currently submitted to NF VALIDATION experts’ review.

Next meetings in water domain:

– NF VALIDATION “Technical Board – Water General protocol” : Friday 11th of March, 2016

NF VALIDATION : 124 methods certified in food microbiology

As of December 03th 2015, 121 alternative methods for microbiological food analysis are certified NF VALIDATION according to the NF EN ISO 16140 standard protocol, and 3 for antibiotic detection in food according to a specific validation protocol.

During its meeting held on December 03rd and 04th 2015, the NF VALIDATION “Technical Board – Food Microbiology” approved by vote:

– The validation according to the new protocol ISO/FDIS 16140-2 (2015) of Thermo Scientific SureTect Cronobacter species PCR Assay (OXOID Thermo Fisher Scienific) for the detection of Cronobacter spp. in infant formula (with or without probiotics) and related industrial environmental samples, and EASY STAPH (SOLABIA) for the enumeration of coagulase-positive Staphylococci in meat products and poultry, dairy products and seafood.

Next meetings of the NF VALIDATION “Technical Board – Food Microbiology”: January 28th and 29th, March 17th and 18th, May 17th and 18th, June 30th and July 1st, September 29th and 30th, November 24th and 25th 2016.