{"id":192,"date":"2014-03-21T11:01:52","date_gmt":"2014-03-21T10:01:52","guid":{"rendered":"http:\/\/nf-validation.afnor.org\/en\/?page_id=192"},"modified":"2026-03-24T16:47:42","modified_gmt":"2026-03-24T15:47:42","slug":"listeria-monocytogenes","status":"publish","type":"page","link":"https:\/\/nf-validation.afnor.org\/en\/food-industry\/listeria-monocytogenes\/","title":{"rendered":"<i>Listeria monocytogenes<\/i>"},"content":{"rendered":"<p>Reference methods: <a title=\"NF EN ISO 11290-1\" href=\"https:\/\/www.boutique.afnor.org\/en-gb\/standard\/nf-en-iso-112901\/microbiology-of-the-food-chain-horizontal-method-for-the-detection-and-enum\/fa151457\/79305\" target=\"_blank\" rel=\"noopener\">NF EN\u00a0ISO 11290-1<\/a> or <a title=\"NF EN ISO 11290-2\" href=\"https:\/\/www.boutique.afnor.org\/en-gb\/standard\/nf-en-iso-112902\/microbiology-of-the-food-chain-horizontal-method-for-the-detection-and-enum\/fa151456\/58977\" target=\"_blank\" rel=\"noopener\">NF EN ISO 11290-2<\/a><\/p>\n<p><em>Section updated and published on March 24th, 2026<\/em><\/p>\n<h2>Culture media<\/h2>\n<p><strong>AL\/Agar Enumeration<\/strong><br \/>\nAim of the method: Enumeration of <i>Listeria monocytogenes <\/i><br \/>\nHolder: BIO-RAD<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/12\/BRD-07-17-01-09_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/\u00a0<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2026\/03\/AL-Enumeration-Lmono-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>COMPASS\u00ae <em>Listeria<\/em> Agar (Enumeration)<\/strong><br \/>\nAim of the method: Enumeration of <i>Listeria monocytogenes <\/i><br \/>\nHolder: SOLABIA S.A.S.<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/06\/BKR-23-05-12-07_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/07\/COMPASS-Listeria-Enumeration-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>Thermo Scientific\u2122 Listeria Precis\u2122 (Detection <em>Listeria monocytogenes<\/em>)<br \/>\n<\/strong> Aim of the method: Detection of <i>Listeria monocytogenes<\/i><br \/>\nHolder: OXOID Ltd, Part of Thermo Fisher Scientific<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/05\/UNI-03-04-04-05-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/10\/Listeria_Precis_L.mono_SR_2025_07_v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>Thermo Scientific\u2122 Listeria Precis\u2122 (Enumeration <em>Listeria monocytogenes<\/em>) <\/strong><br \/>\nAim of the method: Enumeration of <i>Listeria monocytogenes <\/i><br \/>\nHolder: OXOID Ltd, Part of Thermo Fisher Scientific<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/05\/UNI-03-05-09-06-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2014\/03\/Synt-UNI-03-05-09-06_en.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>RAPID&#8217;<em>L. mono<\/em> (Enumeration)<\/strong><br \/>\nAim of the method: Enumeration of <i>Listeria monocytogenes <\/i><br \/>\nHolder: BIO-RAD<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2026\/03\/BRD-07-05-09-01-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/\u00a0<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2026\/02\/RAPID-Lmono-Enumeration-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>One Broth One Plate for <em>Listeria monocytogenes<\/em> (OBOP-LMO)<br \/>\n<\/strong>Aim of the method: Detection of <em>Listeria monocytogenes<\/em><br \/>\nHolder: NEOGEN Corporation<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/03\/NEO-35-06-07-16-en.docx_-1.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/07\/OBOP_L_mono-SR-2024_06-v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<h2>Molecular methods<\/h2>\n<p><strong>Neogen\u00ae Molecular Detection Assay 2 \u2013 <em>Listeria monocytogenes<\/em><br \/>\n<\/strong>Aim of the method: Detection of <em>Listeria\u00a0monocytogenes<\/em><br \/>\nHolder: Neogen Food Safety Corporation<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/12\/3M-01-15-09-16_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/Listeria_MDA2_L_mono_SR_v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>ANSR\u00ae for <em>Listeria monocytogenes<\/em><br \/>\n<\/strong>Aim of the method: Detection of <em>Listeria\u00a0monocytogenes<\/em><br \/>\nHolder: NEOGEN Corporation<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/03\/NEO-35-04-03-16-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/01\/N\u00b004_ANSR_Lmono_2023_12_SR_v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>BACGene <em>Listeria<\/em> <em>monocytogenes<\/em><br \/>\n<\/strong>Aim of the method: Detection of <em>Listeria\u00a0monocytogenes<\/em><br \/>\nHolder: Gold Standard Diagnostics<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2026\/02\/EGS-38-03-01-17-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/wp-content\/uploads\/2024\/03\/N\u00b001_REPORT-BACBot_report_V1.pdf\">Summarized study report (OT-2)<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/02\/N\u00b011_Extension_Study_Report_BACGene_Listeria_Pure2023_V1.pdf\">Summarized study report (software pure)<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/02\/BACGene_Listeria_Multiplex-Lmono-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report (original)<\/a><\/p>\n<p><strong>BAX\u00ae System PCR Assay <em>Listeria monocytogenes<\/em> 24E<br \/>\n<\/strong> Aim of the method: Detection of <i>Listeria monocytogenes<\/i><br \/>\nHolder: Hygiena LLC<br \/>\n<a title=\"Certificate\" href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/03\/QUA-18-05-07-08-en.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a title=\"Summarized study report\" href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/07\/BAX-PCR-Lmono-24E-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>GENE-UP\u00ae\u00a0<em>Listeria<\/em>\u00a0<em>monocytogenes<\/em><br \/>\n<\/strong> Aim of the method: Detection of <em>Listeria\u00a0monocytogenes<\/em><br \/>\nHolder:\u00a0bioM\u00e9rieux<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/10\/BIO-12-40-11-16_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/GENE_UP_LMO_Summary_Report_10_2024.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>iQ-Check\u00ae <em>Listeria monocytogenes<\/em> II<br \/>\n<\/strong> Aim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder: BIO-RAD<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/05\/BRD-07-10-04-05_en-.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/09\/iQ-Check-lmono-II-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>MicroSEQ\u00ae <em>Listeria monocytogenes<\/em><\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder: Life Technologies Corporation, Part of Thermo Fisher Scientific<br \/>\n<a title=\"Certificate\" href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/08\/ABI-29-05-12-11-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/\u00a0<a title=\"Summarized study report\" href=\"https:\/\/nf-validation.afnor.org\/wp-content\/uploads\/2023\/12\/N\u00b023_MicroSEQ-Lmono-SUMMARY-REPORT-Version-0.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>Thermo Scientific\u2122 SureTect\u2122 Listeria monocytogenes PCR Assay <\/strong><br \/>\nAim of the method: Detection of <i>Listeria<\/i> <em>monocytogenes<\/em><br \/>\nHolder: OXOID Ltd, Part of Thermo Fisher Scientific<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/10\/UNI-03-08-11-13-en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a> \/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/11\/2025_10_SureTect_Listeria_mono_SR-v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>BAX\u00ae System RT PCR Assay <em>Listeria monocytogenes<\/em><\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes\u00a0<\/i><br \/>\nHolder: Hygiena LLC<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/03\/QUA-18-10-01-19_en.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2022\/12\/Synt-QUA-18-10-01-19.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<h2>Immunological tests<\/h2>\n<p><strong>Solus <em>Listeria monocytogenes <\/em>ELISA<\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder: SOLUS Scientific Ltd. (part of Perkinekmer)<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/04\/SOL-37-05-10-22-en.pdf\" target=\"_blank\" rel=\"noopener\">Certificat<\/a> \/<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2022\/12\/Synt-SOL-37-05-10-22.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>VIDAS\u00ae <em>Listeria monocytogenes<\/em> 2 (LMO2 &#8211; with enrichment step at 30\u00b0C)<\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder:\u00a0bioM\u00e9rieux<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/04\/BIO-12-09-07-02-en-.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\u00a0\/ <a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2014\/03\/Synt-BIO-12-09-07-02_en.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>VIDAS\u00ae <em>Listeria monocytogenes<\/em> 2 (LMO2 &#8211; with enrichment step at 37\u00b0C)<\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder:\u00a0bioM\u00e9rieux<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/04\/BIO-12-11-03-04_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\/ <a title=\"Summarized study report\" href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2024\/03\/VIDAS_LMO2_37_2024_03_SR_v0-protected.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><strong>VIDAS\u00ae <em>Listeria monocytogenes<\/em> Xpress (VIDAS LMX)<\/strong><br \/>\nAim of the method: Detection of <i>Listeria monocytogenes <\/i><br \/>\nHolder: bioM\u00e9rieux<br \/>\n<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2025\/12\/BIO-12-27-02-10_en.docx_.pdf\" target=\"_blank\" rel=\"noopener\">Certificate<\/a>\/<a href=\"https:\/\/nf-validation.afnor.org\/en\/wp-content\/uploads\/sites\/2\/2014\/03\/Synt-BIO-12-27-02-10_en.pdf\" target=\"_blank\" rel=\"noopener\">Summarized study report<\/a><\/p>\n<p><a class=\"btn-blue\" href=\"\/en\/food-industry\/\">Back to the list<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Reference methods: NF EN\u00a0ISO 11290-1 or NF EN ISO 11290-2 Section updated and published on March 24th, 2026 Culture media AL\/Agar Enumeration Aim of the&#8230;<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":50,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-192","page","type-page","status-publish","hentry","post-item","post-even"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.8 - 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